Hands On

25th October 2022: "Hands on exploring methodologies for EV translational research and biomarker detection"

 

Cells continuously secrete membrane vesicles to the extracellular space, which are called exosomes, microvesicles, apoptotic bodies, exomeres, etc… based on their biogenesis origin and size. Extracellular Vesicles  (EVs) have characteristic molecular cargo (protein, RNA, DNA, etc…) composition that partially depends on the cell of origin, carrying bioactive components that can influence the behaviour of target cells upon EV uptake. Therefore, EVs have been proposed as mediators of cell-to-cell communication in multiple biological and pathological processes such as coagulation, angiogenesis and tumor progression.

 

The fact that EVs can be detected in many biological fluids, including blood, saliva, urine and milk, makes them great candidates as non-invasive biomarkers for the diagnosis of different pathological processes such as chronic inflammation, cancer, cardiovascular diseases or infectious diseases.

 

In this hands-on workshop, we will summarize the state of the art in EV research as disease biomarkers, their role in physiology and pathology as well as the main technological tools we currently have for their study and characterization. Several hands-on demonstrations will be performed on October 25th in groups so that the attendants can know by first-hand the main resources devoted to EV analyses that are currently in the market.


There is a limit of 50 places for the workshop.